Web非小细胞肺癌A549细胞中驱动蛋白KIF4A的表达量极低.为研究KIF4A在肺癌发生和发展过程中的作用,利用脂质体转染的方法,将pEGFP-KIF4A质粒和pEGFP-C1质粒分别转入A549细胞中,用G418筛选得到单克隆,并扩增得到稳定表达GFP-KIF4A和GFP的细胞株.利用Western Blot以及免疫荧光染色法对得到的细胞株进行鉴定.结果表明 ... Web细胞生长曲线是测定细胞生长数的常用方法,也是判定细胞活力的重要指标,是培养细胞生物学特性的基本参数之一。 图示四种细胞在使用F102血清进行培养后,既定时间内均能达到标准密度,并且使用F102进行培养的细胞增殖能力明显优于Supplier A,可媲美甚至在 ...
CGA衍生多肽CHR抑制肿瘤坏死因子-α诱导血管内皮细胞高渗透性 …
http://www.koovin.com/?a=url&id=12808478 Web2.4 蛋白免疫印迹实验(Western blotting) 取对数生长期的EA.hy926细胞,以 5×108/L的密度接种于6孔板中,待细胞贴壁后加入相应处理因素,处理24 h后 用RIPA裂解液提取细胞蛋白,BCA法测蛋白浓度,SDS-PAGE电泳每孔上样50 μg 的蛋白后经转膜、封闭,LC3-Ⅱ抗体(1∶1 000)和羊 … imx events swimming
一种多功能高效药物递送系统及其制备方法和应用【掌桥专利】
WebJun 27, 2013 · The glass slides were cleaved into 1 cm x 1 cm plates, rinsed both sides with ethanol and exposed to UV light in laminar chamber for 30 min in order to sterilize. Next, EA.hy926 cells were seeded on the glass slides at density of 10 4 cells/ml, and cultured to 80% confluence. Afterwards, the sample was immediately taken for measurements. Web描述. Under PEG stress, the primary cultured human umbilical vein cells were fused with the A549 clone of anti-thioguanine to construct the human umbilical vein cell line, EA.hy926. Fusions are grown on HAT medium and screened for eight-factor-associated antigens. EA.hy926 has undergone more than 100 population doubling (PDLs). WebEA.hy926细胞ATCC CRL-2922人脐静脉细胞融合细胞特点和简介 在PEG胁迫下,将原代培养的人脐静脉细胞与抗硫鸟嘌呤的A549克隆株融合,构建了人脐静脉细胞株, EA.hy926 … in250a是什么意思